| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Mesomycoplasma hyopneumoniae | TGGAATTGGAAAATCGCTGGC | TTCCGGTGGCGAATTCCA | 59.80 | 59.25 | 249 | 102 |
100.00%
|
100.00%
|
| Primer 42 | Mesomycoplasma hyopneumoniae | AATCGCCTTCGCAAGTGC | CACGCACCCTTGGAATTGC | 59.13 | 60.08 | 270 | 101 |
100.00%
|
100.00%
|
| Primer 43 | Mesomycoplasma hyopneumoniae | AATCGCCTTCGCAAGTGC | ACGGAAATCACGCACCCT | 59.13 | 59.25 | 278 | 100 |
100.00%
|
100.00%
|
| Primer 44 | Mesomycoplasma hyopneumoniae | TGCGAGGATTCACGGGTT | CAAATGCGCAAGGTCCTGG | 58.93 | 59.79 | 253 | 100 |
100.00%
|
100.00%
|
| Primer 45 | Mesomycoplasma hyopneumoniae | TCGGCAATTAGAAATCGCAGT | TCGGCTAGATTTTCGCCTAGT | 58.37 | 58.97 | 186 | 99 |
100.00%
|
100.00%
|
| Primer 46 | Mesomycoplasma hyopneumoniae | TCGGCAATTAGAAATCGCAGT | CGGCTAGATTTTCGCCTAGTC | 58.37 | 58.60 | 185 | 99 |
100.00%
|
100.00%
|
| Primer 47 | Mesomycoplasma hyopneumoniae | TCAAACCGTGAAGACAAGCAA | TCTCGCAGGGCATTTGCA | 58.64 | 59.97 | 284 | 97 |
100.00%
|
100.00%
|
| Primer 48 | Mesomycoplasma hyopneumoniae | AGCGCAATTGTAGCTGCAA | TCTTGTCGGATCAGACGCA | 58.74 | 58.74 | 164 | 97 |
100.00%
|
100.00%
|
| Primer 49 | Mesomycoplasma hyopneumoniae | AAGCGCAATTGTAGCTGCAA | TCTTGTCGGATCAGACGCA | 59.40 | 58.74 | 165 | 97 |
100.00%
|
100.00%
|
| Primer 50 | Mesomycoplasma hyopneumoniae | ATGCGAGGATTCACGGGT | CAAATGCGCAAGGTCCTGG | 58.69 | 59.79 | 254 | 95 |
100.00%
|
100.00%
|